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Theoretical and practical course on gene expression analysis for improved genotoxicity hazard assessment using GENOMARK

Theoretical and practical course on gene expression analysis for improved genotoxicity hazard assessment using GENOMARK

We are pleased to invite you to take part in the S&T course
“TWINALT – Genotoxicity: Theoretical and practical course on gene expression analysis for improved genotoxicity hazard assessment using GENOMARK”
Organized by Vrije Universiteit Brussel (VUB)

 

This is an on site event dedicated for researchers from partner institutions (max. 3 students*; *vaccination is mandatory to enter the laboratory)

Duration: January 17th  – February 11th , 2022 (4 weeks)

Location: VUB Health Campus, Dept. In Vitro Toxicology and Dermato-cosmetology, Brussels

Submission Due: November 15th, 2021

 

Genetic toxicity testing is routinely performed to ensure the safety of newly developed chemicals for human health. Traditionally, a step-wise standardized approach is applied, starting with a battery of in vitro tests covering both gene mutations as well as structural and numerical chromosome aberrations. In case of a positive outcome in one of the in vitro tests, an adequate in vivo follow-up test is performed. Despite its wide applicability and high sensitivity, the current genotoxicity battery is facing several limitations including, the lack of information on the underlying mode of action (MoA) and the high number of misleading positive results. These “misleading positives” are chemicals with a positive result in at least one of the in vitro tests but a negative result in the associated follow-up in vivo test and are caused by the low specificity of the in vitro genotoxicity tests. As the misleading positive results trigger needless animal studies, which are costly, time-consuming and not always biologically relevant to humans, the existing genotoxicity testing strategies need to be improved. One interesting domain of new approach methodologies (NAMs) includes gene expression biomarkers. We developed the gene signature GENOMARK, consisting of 84 biomarker genes to identify genotoxic substances in metabolically competent human HepaRGTM cells. Gene expression is measured using qPCR array. After an introduction to genotoxicity, the course will focus on the presentation and discussion on the application and interpretation of the GENOMARK tool. Practical training sessions will be given to teach the cultivation of HepaRGTM cells, determination of non-cytotoxic concentrations of compounds, exposure of the cells to the compounds, preparation of the samples for gene expression analysis, gene expression analysis (qPCR) and data analysis using the R software.

More information, please send an e-mail to: Anouck Thienpont:  Anouck.Ingrid.Thienpont@vub.be

For registration*, send an email to: Anouck Thienpont:  Anouck.Ingrid.Thienpont@vub.be

* After the course all participants will be asked for a short presentation during the Workshop of Return Mechanism 2, which will take place during Consortium Event 3 in October 2022. By taking part in the course the participants agree to record and publish YouTube videos and other documentations on social media related to the course on the TWINALT website.

Registration form for the event